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sigmoid 4pl interpolation graphpad prism 9.0  (GraphPad Software Inc)


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    GraphPad Software Inc sigmoid 4pl interpolation graphpad prism 9.0
    A) The duration of anti-SARS-CoV-2 RBD (endpoint titers) in the i.n. immunized mice sera after booster immunization (3wpB and 5wpB) were measured. B) The neutralization titers (50% inhibitory dose, ID 50 ) in immunized mice sera (3wpB) against pseudovirus PV-Luc-SP Delta infection were determined. The serially diluted mouse sera were incubated with PV-Luc-SP Delta (∼10 4 RLU) and then, the mixtures (PV + Sera) were used to inoculate A549 ACE2 cells. The infection of PV was determined by luciferase assay at 48∼66 hrs post-infection. The percentage of infection was calculated compared with no serum control. The 50% inhibition dose (ID 50 ) neutralizing Ab titers were calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0, as described in Materials and Methods. C) The duration of neutralizing Ab ID 50 titers in the i.n. immunized mice sera collected at 3wpB and 5wpB. Data represent mean ±SEM. Statistical significance was determined using one-way ANOVA test and Tukey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. wpB, week post Boost.
    Sigmoid 4pl Interpolation Graphpad Prism 9.0, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sigmoid+4pl+interpolation+graphpad+prism+9%2E0/sigmoid+4pl+interpolation+graphpad+prism+9+0/bio_rxiv__2023__07__14__549076-172-12-11
    Average 90 stars, based on 1 article reviews
    sigmoid 4pl interpolation graphpad prism 9.0 - by Bioz Stars, 2026-10
    90/100 stars

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    1) Product Images from "Oral immunization with rVSV bivalent vaccine elicits protective immune responses, including ADCC, against both SARS-CoV-2 and Influenza A viruses"

    Article Title: Oral immunization with rVSV bivalent vaccine elicits protective immune responses, including ADCC, against both SARS-CoV-2 and Influenza A viruses

    Journal: bioRxiv

    doi: 10.1101/2023.07.14.549076

    A) The duration of anti-SARS-CoV-2 RBD (endpoint titers) in the i.n. immunized mice sera after booster immunization (3wpB and 5wpB) were measured. B) The neutralization titers (50% inhibitory dose, ID 50 ) in immunized mice sera (3wpB) against pseudovirus PV-Luc-SP Delta infection were determined. The serially diluted mouse sera were incubated with PV-Luc-SP Delta (∼10 4 RLU) and then, the mixtures (PV + Sera) were used to inoculate A549 ACE2 cells. The infection of PV was determined by luciferase assay at 48∼66 hrs post-infection. The percentage of infection was calculated compared with no serum control. The 50% inhibition dose (ID 50 ) neutralizing Ab titers were calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0, as described in Materials and Methods. C) The duration of neutralizing Ab ID 50 titers in the i.n. immunized mice sera collected at 3wpB and 5wpB. Data represent mean ±SEM. Statistical significance was determined using one-way ANOVA test and Tukey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. wpB, week post Boost.
    Figure Legend Snippet: A) The duration of anti-SARS-CoV-2 RBD (endpoint titers) in the i.n. immunized mice sera after booster immunization (3wpB and 5wpB) were measured. B) The neutralization titers (50% inhibitory dose, ID 50 ) in immunized mice sera (3wpB) against pseudovirus PV-Luc-SP Delta infection were determined. The serially diluted mouse sera were incubated with PV-Luc-SP Delta (∼10 4 RLU) and then, the mixtures (PV + Sera) were used to inoculate A549 ACE2 cells. The infection of PV was determined by luciferase assay at 48∼66 hrs post-infection. The percentage of infection was calculated compared with no serum control. The 50% inhibition dose (ID 50 ) neutralizing Ab titers were calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0, as described in Materials and Methods. C) The duration of neutralizing Ab ID 50 titers in the i.n. immunized mice sera collected at 3wpB and 5wpB. Data represent mean ±SEM. Statistical significance was determined using one-way ANOVA test and Tukey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. wpB, week post Boost.

    Techniques Used: Neutralization, Infection, Incubation, Luciferase, Inhibition

    Related Articles

    Neutralization:

    Article Title: Oral immunization with rVSV bivalent vaccine elicits protective immune responses, including ADCC, against both SARS-CoV-2 and Influenza A viruses
    Article Snippet: (ID 50 ) were defined as the reciprocal of the serum maximum dilution that reduced RLUs by 50% compared with no-serum (virus and cell) controls. .. The ID 50 was calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0.

    Infection:

    Article Title: Oral immunization with rVSV bivalent vaccine elicits protective immune responses, including ADCC, against both SARS-CoV-2 and Influenza A viruses
    Article Snippet: (ID 50 ) were defined as the reciprocal of the serum maximum dilution that reduced RLUs by 50% compared with no-serum (virus and cell) controls. .. The ID 50 was calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0.

    Incubation:

    Article Title: Oral immunization with rVSV bivalent vaccine elicits protective immune responses, including ADCC, against both SARS-CoV-2 and Influenza A viruses
    Article Snippet: (ID 50 ) were defined as the reciprocal of the serum maximum dilution that reduced RLUs by 50% compared with no-serum (virus and cell) controls. .. The ID 50 was calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0.

    Luciferase:

    Article Title: Oral immunization with rVSV bivalent vaccine elicits protective immune responses, including ADCC, against both SARS-CoV-2 and Influenza A viruses
    Article Snippet: (ID 50 ) were defined as the reciprocal of the serum maximum dilution that reduced RLUs by 50% compared with no-serum (virus and cell) controls. .. The ID 50 was calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0.

    Inhibition:

    Article Title: Oral immunization with rVSV bivalent vaccine elicits protective immune responses, including ADCC, against both SARS-CoV-2 and Influenza A viruses
    Article Snippet: (ID 50 ) were defined as the reciprocal of the serum maximum dilution that reduced RLUs by 50% compared with no-serum (virus and cell) controls. .. The ID 50 was calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0.



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    sigmoid 4pl interpolation graphpad prism 9.0  (GraphPad Software Inc)
    90
    GraphPad Software Inc sigmoid 4pl interpolation graphpad prism 9.0
    A) The duration of anti-SARS-CoV-2 RBD (endpoint titers) in the i.n. immunized mice sera after booster immunization (3wpB and 5wpB) were measured. B) The neutralization titers (50% inhibitory dose, ID 50 ) in immunized mice sera (3wpB) against pseudovirus PV-Luc-SP Delta infection were determined. The serially diluted mouse sera were incubated with PV-Luc-SP Delta (∼10 4 RLU) and then, the mixtures (PV + Sera) were used to inoculate A549 ACE2 cells. The infection of PV was determined by luciferase assay at 48∼66 hrs post-infection. The percentage of infection was calculated compared with no serum control. The 50% inhibition dose (ID 50 ) neutralizing Ab titers were calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0, as described in Materials and Methods. C) The duration of neutralizing Ab ID 50 titers in the i.n. immunized mice sera collected at 3wpB and 5wpB. Data represent mean ±SEM. Statistical significance was determined using one-way ANOVA test and Tukey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. wpB, week post Boost.
    Sigmoid 4pl Interpolation Graphpad Prism 9.0, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sigmoid+4pl+interpolation+graphpad+prism+9%2E0/sigmoid+4pl+interpolation+graphpad+prism+9+0/bio_rxiv__2023__07__14__549076-172-12-11
    Average 90 stars, based on 1 article reviews
    sigmoid 4pl interpolation graphpad prism 9.0 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

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    A) The duration of anti-SARS-CoV-2 RBD (endpoint titers) in the i.n. immunized mice sera after booster immunization (3wpB and 5wpB) were measured. B) The neutralization titers (50% inhibitory dose, ID 50 ) in immunized mice sera (3wpB) against pseudovirus PV-Luc-SP Delta infection were determined. The serially diluted mouse sera were incubated with PV-Luc-SP Delta (∼10 4 RLU) and then, the mixtures (PV + Sera) were used to inoculate A549 ACE2 cells. The infection of PV was determined by luciferase assay at 48∼66 hrs post-infection. The percentage of infection was calculated compared with no serum control. The 50% inhibition dose (ID 50 ) neutralizing Ab titers were calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0, as described in Materials and Methods. C) The duration of neutralizing Ab ID 50 titers in the i.n. immunized mice sera collected at 3wpB and 5wpB. Data represent mean ±SEM. Statistical significance was determined using one-way ANOVA test and Tukey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. wpB, week post Boost.

    Journal: bioRxiv

    Article Title: Oral immunization with rVSV bivalent vaccine elicits protective immune responses, including ADCC, against both SARS-CoV-2 and Influenza A viruses

    doi: 10.1101/2023.07.14.549076

    Figure Lengend Snippet: A) The duration of anti-SARS-CoV-2 RBD (endpoint titers) in the i.n. immunized mice sera after booster immunization (3wpB and 5wpB) were measured. B) The neutralization titers (50% inhibitory dose, ID 50 ) in immunized mice sera (3wpB) against pseudovirus PV-Luc-SP Delta infection were determined. The serially diluted mouse sera were incubated with PV-Luc-SP Delta (∼10 4 RLU) and then, the mixtures (PV + Sera) were used to inoculate A549 ACE2 cells. The infection of PV was determined by luciferase assay at 48∼66 hrs post-infection. The percentage of infection was calculated compared with no serum control. The 50% inhibition dose (ID 50 ) neutralizing Ab titers were calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0, as described in Materials and Methods. C) The duration of neutralizing Ab ID 50 titers in the i.n. immunized mice sera collected at 3wpB and 5wpB. Data represent mean ±SEM. Statistical significance was determined using one-way ANOVA test and Tukey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. wpB, week post Boost.

    Article Snippet: The ID 50 was calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0.

    Techniques: Neutralization, Infection, Incubation, Luciferase, Inhibition